Study on serum - free medium for 5b1 cell growth 1細(xì)胞生長的無血清培養(yǎng)基的研究
Methods : cell culture in serum - free medium , indirect immunofluorescence cytochemistry were used 方法:采用無血清細(xì)胞培養(yǎng)技術(shù),間接免疫細(xì)胞化學(xué)染色法。
We report a novel three - stage method to efficiently direct the differentiation of human embryonic stem cells into hepatic cells in serum - free medium 我們報道一種新的三步法有效誘導(dǎo)人胚胎干細(xì)胞在無血漿培養(yǎng)液中向肝細(xì)胞分化。
The stable expression cell line was selected . expression level of the line cultured in serum - free medium was 580iu 106 cell 24h . the product expressed was purified by zn 篩選到的穩(wěn)定表達(dá)株在無血清培養(yǎng)基的表達(dá)量為580iu 106細(xì)胞24h 。
Materials and mehods nscs were isolated from e - 14 embryonic rat ' s brains , then cultured in serum - free medium with egf and bfgf ( 20ng / ml respectively ) as mitogens 12小時至36小時之間,死亡率上升最快: 36一48小時,死亡率幾乎不再上升,穩(wěn)定在60 %左右。
However , the drawbacks of mammalian cell expression systems are apparent : they are difficult to culture in large scale , especially in serum - free medium ; the productivity is much lower than other expression systems and the costs are higher 但哺乳動物細(xì)胞表達(dá)系統(tǒng)的缺點(diǎn)也很明顯,如表達(dá)量低、大規(guī)模培養(yǎng)困難、生產(chǎn)成本高昂等。
Development of insect cell culture media and serum - free media , establishment of insect cell lines and applications of insect cell culture for production of biopesticides and expression of recombinant proteins in genetic engineering were reviewed in this paper 摘要綜述了昆蟲細(xì)胞培養(yǎng)基的現(xiàn)狀和發(fā)展、無血清培養(yǎng)基的開發(fā)、昆蟲細(xì)胞系的建立以及昆蟲細(xì)胞在生物農(nóng)藥和重組蛋白中的應(yīng)用。
Results the epithelial cells cultured in serum - free medium not only demonstrated a significant increase in proliferative , colony - forming efficiency and cell generations , but also have the normal structural and functional properties than those in serum - containing medium 結(jié)果無血清培養(yǎng)系統(tǒng)中結(jié)膜上皮細(xì)胞的增殖、克隆形成及細(xì)胞傳代能力均較含血清培養(yǎng)系統(tǒng)高,而且具備結(jié)膜上皮細(xì)胞全部組織結(jié)構(gòu)及功能特點(diǎn)。
Thus , the success of nscs therapy is determined by the induced differentiation and the effect of micro - enviroment on the survival , proliferation and differentiation of it . the isolation and culture of nscs in vitro successfully is the presupposition for its research . to avoid the influence of unknown factors exiting in serum on nscs " differentiation , serum - free medium with egf and bfgf as mitogens to stimulate proliferation and inhibit differentiation in vitro was introduced through a long period of search 淀粉樣蛋白( amyloidpeptidy , a )是淀粉樣蛋白前體蛋白( amyloidprecusorprotein , app )異常代謝產(chǎn)生的含有39 ? 43個氨基酸殘基的疏水肽,可聚合成不溶的纖維形式或不定型顆粒狀結(jié)構(gòu),導(dǎo)致各種毒性反應(yīng),在阿爾茨海默病( alzheimer ' sdisease , ad )發(fā)病中起著至關(guān)重要的作用。
Subconfluent cultures of rat were maintained in dmem / 10 % fbs , 24hrs before neuronal induction , media were replaced with pre - induction media consisting of dmem / 10 % fbs / lmm beta - mercaptoethanol ( bme ) , to initiate neuronal differentiation , the preinduction media were removed , and the cells were washed with pbs and transferred to neuronal induction media composed of dmem / serum - free media , 5hrs later , 40ul dmso was given to every hole containing 2ml each 分別取第5代和第13代的mscs ,以8x10 cm ‘濃度接種于六孔板中的蓋玻片上,制備細(xì)胞爬片,每孔加zinl培養(yǎng)液。達(dá)到80融合時,更換新鮮培養(yǎng)液,并在培養(yǎng)液中加人終濃度為lrnm的p一流基乙醇,誘導(dǎo)24小時, pbs洗滌,而后換成無血清的培養(yǎng)液。